PoplarV1, Main, Exploration, bibRecord, 004243

Isolation of high-quality RNA from gymnosperm and angiosperm trees.

Identifieur interne : 004243 ( Main/Exploration ); précédent : 004242; suivant : 004244

Isolation of high-quality RNA from gymnosperm and angiosperm trees.

Auteurs : Natalia Kolosova [Canada] ; Barbara Miller ; Steven Ralph ; Brian E. Ellis ; Carl Douglas ; Kermit Ritland ; Jörg Bohlmann

Source :

BioTechniques [ 0736-6205 ] ; 2004.

RBID : pubmed:15152602

Descripteurs français

KwdFr :
- ARN des plantes (isolement et purification), Cycadopsida (génétique), Fractionnement chimique (méthodes), Magnoliopsida (génétique), Manipulation d'échantillons (méthodes), Mélanges complexes (analyse).
MESH :
- analyse : Mélanges complexes.
- génétique : Cycadopsida, Magnoliopsida.
- isolement et purification : ARN des plantes.
- méthodes : Fractionnement chimique, Manipulation d'échantillons.

English descriptors

KwdEn :
- Chemical Fractionation (methods), Complex Mixtures (analysis), Cycadopsida (genetics), Magnoliopsida (genetics), RNA, Plant (isolation & purification), Specimen Handling (methods).
MESH :
- chemical , analysis : Complex Mixtures.
- genetics : Cycadopsida, Magnoliopsida.
- chemical , isolation & purification : RNA, Plant.
- methods : Chemical Fractionation, Specimen Handling.

Abstract

An improved protocol was developed for efficient and reliable extraction of high-quality total RNA and mRNA from various tissues of spruce (Picea spp.) and poplar (Populus spp.) trees, as well as other plant species. This method was specifically optimized for tissues with high content of polysaccharides, oleoresin terpenoids, and phenolic secondary metabolites, which often co-precipitate with RNA and inhibit subsequent reverse transcription. The improved protocol yielded up to 600 micrograms of total RNA per gram of tissue suitable for standard expressed sequence tags (ESTs), full-length cDNA library construction, and for microarray applications.

DOI: 10.2144/04365ST06
PubMed: 15152602

Affiliations:

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Le document en format XML

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<author><name sortKey="Kolosova, Natalia" sort="Kolosova, Natalia" uniqKey="Kolosova N" first="Natalia" last="Kolosova">Natalia Kolosova</name>
<affiliation wicri:level="4"><nlm:affiliation>University of British Columbia, Vancouver, BC, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>University of British Columbia, Vancouver, BC</wicri:regionArea>
<orgName type="university">Université de la Colombie-Britannique</orgName>
<placeName><settlement type="city">Vancouver</settlement>
<region type="state">Colombie-Britannique </region>
</placeName>
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<author><name sortKey="Miller, Barbara" sort="Miller, Barbara" uniqKey="Miller B" first="Barbara" last="Miller">Barbara Miller</name>
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<author><name sortKey="Ralph, Steven" sort="Ralph, Steven" uniqKey="Ralph S" first="Steven" last="Ralph">Steven Ralph</name>
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<author><name sortKey="Ellis, Brian E" sort="Ellis, Brian E" uniqKey="Ellis B" first="Brian E" last="Ellis">Brian E. Ellis</name>
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<author><name sortKey="Douglas, Carl" sort="Douglas, Carl" uniqKey="Douglas C" first="Carl" last="Douglas">Carl Douglas</name>
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<author><name sortKey="Ritland, Kermit" sort="Ritland, Kermit" uniqKey="Ritland K" first="Kermit" last="Ritland">Kermit Ritland</name>
</author>
<author><name sortKey="Bohlmann, Jorg" sort="Bohlmann, Jorg" uniqKey="Bohlmann J" first="Jörg" last="Bohlmann">Jörg Bohlmann</name>
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<author><name sortKey="Bohlmann, Jorg" sort="Bohlmann, Jorg" uniqKey="Bohlmann J" first="Jörg" last="Bohlmann">Jörg Bohlmann</name>
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<term>Complex Mixtures (analysis)</term>
<term>Cycadopsida (genetics)</term>
<term>Magnoliopsida (genetics)</term>
<term>RNA, Plant (isolation & purification)</term>
<term>Specimen Handling (methods)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>ARN des plantes (isolement et purification)</term>
<term>Cycadopsida (génétique)</term>
<term>Fractionnement chimique (méthodes)</term>
<term>Magnoliopsida (génétique)</term>
<term>Manipulation d'échantillons (méthodes)</term>
<term>Mélanges complexes (analyse)</term>
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<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Complex Mixtures</term>
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<term>Magnoliopsida</term>
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<term>Specimen Handling</term>
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<front><div type="abstract" xml:lang="en">An improved protocol was developed for efficient and reliable extraction of high-quality total RNA and mRNA from various tissues of spruce (Picea spp.) and poplar (Populus spp.) trees, as well as other plant species. This method was specifically optimized for tissues with high content of polysaccharides, oleoresin terpenoids, and phenolic secondary metabolites, which often co-precipitate with RNA and inhibit subsequent reverse transcription. The improved protocol yielded up to 600 micrograms of total RNA per gram of tissue suitable for standard expressed sequence tags (ESTs), full-length cDNA library construction, and for microarray applications.</div>
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<Issue>5</Issue>
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<Month>May</Month>
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<Title>BioTechniques</Title>
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<ArticleTitle>Isolation of high-quality RNA from gymnosperm and angiosperm trees.</ArticleTitle>
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<Abstract><AbstractText>An improved protocol was developed for efficient and reliable extraction of high-quality total RNA and mRNA from various tissues of spruce (Picea spp.) and poplar (Populus spp.) trees, as well as other plant species. This method was specifically optimized for tissues with high content of polysaccharides, oleoresin terpenoids, and phenolic secondary metabolites, which often co-precipitate with RNA and inhibit subsequent reverse transcription. The improved protocol yielded up to 600 micrograms of total RNA per gram of tissue suitable for standard expressed sequence tags (ESTs), full-length cDNA library construction, and for microarray applications.</AbstractText>
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Isolation of high-quality RNA from gymnosperm and angiosperm trees.

Isolation of high-quality RNA from gymnosperm and angiosperm trees.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki